Diversity of legionellae strains from Tunisian hot spring water.
Identifieur interne : 000188 ( Main/Exploration ); précédent : 000187; suivant : 000189Diversity of legionellae strains from Tunisian hot spring water.
Auteurs : Taoufik Ghrairi [Tunisie] ; Nawel Chaftar ; Sophie Jarraud ; Jean Marc Berjeaud ; Khaled Hani ; Jacques FrereSource :
- Research in microbiology [ 1769-7123 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN bactérien (composition chimique), ADN bactérien (génétique), ADN ribosomique (composition chimique), ADN ribosomique (génétique), ARN ribosomique 16S (génétique), Analyse de regroupements (MeSH), Analyse de séquence d'ADN (MeSH), Biodiversité (MeSH), Données de séquences moléculaires (MeSH), Legionella (classification), Legionella (croissance et développement), Legionella (génétique), Legionella (isolement et purification), Phylogenèse (MeSH), Réaction de polymérisation en chaine en temps réel (MeSH), Sources thermales (microbiologie), Sérotypie (MeSH), Techniques de typage bactérien (MeSH), Température élevée (MeSH), Tunisie (MeSH), Typage moléculaire (MeSH), Électrophorèse en champ pulsé (MeSH).
- MESH :
- composition chimique : ADN bactérien, ADN ribosomique, Legionella.
- croissance et développement : Legionella.
- génétique : ADN bactérien, ADN ribosomique, ARN ribosomique 16S, Legionella.
- isolement et purification : Legionella.
- microbiologie : Sources thermales.
- Analyse de regroupements, Analyse de séquence d'ADN, Biodiversité, Données de séquences moléculaires, Phylogenèse, Réaction de polymérisation en chaine en temps réel, Sérotypie, Techniques de typage bactérien, Température élevée, Tunisie, Typage moléculaire, Électrophorèse en champ pulsé.
- Wicri :
- geographic : Tunisie.
English descriptors
- KwdEn :
- Bacterial Typing Techniques (MeSH), Biodiversity (MeSH), Cluster Analysis (MeSH), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Electrophoresis, Gel, Pulsed-Field (MeSH), Hot Springs (microbiology), Hot Temperature (MeSH), Legionella (classification), Legionella (genetics), Legionella (growth & development), Legionella (isolation & purification), Molecular Sequence Data (MeSH), Molecular Typing (MeSH), Phylogeny (MeSH), RNA, Ribosomal, 16S (genetics), Real-Time Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH), Serotyping (MeSH), Tunisia (MeSH).
- MESH :
- chemical , chemistry : DNA, Bacterial, DNA, Ribosomal.
- chemical , genetics : DNA, Bacterial, DNA, Ribosomal, RNA, Ribosomal, 16S.
- geographic : Tunisia.
- classification : Legionella.
- genetics : Legionella.
- growth & development : Legionella.
- isolation & purification : Legionella.
- microbiology : Hot Springs.
- Bacterial Typing Techniques, Biodiversity, Cluster Analysis, Electrophoresis, Gel, Pulsed-Field, Hot Temperature, Molecular Sequence Data, Molecular Typing, Phylogeny, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Serotyping.
Abstract
The aim of this study was to evaluate the frequency of Legionella pneumophila in three hot spring water recreation areas by conventional culture on GVPC and by Real Time-PCR. No legionellae were isolated from the spring water upstream the distribution system suggesting strongly that the thermal area was the probable source of these pathogens. L. pneumophila was present at high level in some samples, reaching values of 8.2 × 10(3) CFU/l at the site C. We observed a low diversity among the 18 isolated Legionella strains according to 16S DNA analysis, since only L. pneumophila and Legionella londiniensis were identified. All of the L. pneumophila strains belong to five serogroups (1, 4, 5, 6 and 8). Pulsed-field gel electrophoresis analysis identified various genotypes among these serogroups and showed that these strains had pulsotypes distinct from L. pneumophila present in the CNRL Legionella-database. These environmental strains showed higher tolerance to heat shock stress at 48 °C than L. pneumophila clinical strain, which may explain their presence in warm waters. Our data suggested that potential pathogen legionellae are present in Tunisian medicalized thermal spas and emphasized the importance of adopting control measures to prevent infection.
DOI: 10.1016/j.resmic.2013.01.002
PubMed: 23385143
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000632
- to stream PubMed, to step Curation: 000628
- to stream PubMed, to step Checkpoint: 000625
- to stream Main, to step Merge: 000188
- to stream Main, to step Curation: 000188
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Diversity of legionellae strains from Tunisian hot spring water.</title><author><name sortKey="Ghrairi, Taoufik" sort="Ghrairi, Taoufik" uniqKey="Ghrairi T" first="Taoufik" last="Ghrairi">Taoufik Ghrairi</name><affiliation wicri:level="1"><nlm:affiliation>UR08-45, Department of Biochemistry, Faculty of Medicine of Sousse, 4002 Sousse, Tunisia. taoufik_ghrairi@yahoo.fr</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>UR08-45, Department of Biochemistry, Faculty of Medicine of Sousse, 4002 Sousse</wicri:regionArea><wicri:noRegion>4002 Sousse</wicri:noRegion></affiliation></author><author><name sortKey="Chaftar, Nawel" sort="Chaftar, Nawel" uniqKey="Chaftar N" first="Nawel" last="Chaftar">Nawel Chaftar</name></author><author><name sortKey="Jarraud, Sophie" sort="Jarraud, Sophie" uniqKey="Jarraud S" first="Sophie" last="Jarraud">Sophie Jarraud</name></author><author><name sortKey="Berjeaud, Jean Marc" sort="Berjeaud, Jean Marc" uniqKey="Berjeaud J" first="Jean Marc" last="Berjeaud">Jean Marc Berjeaud</name></author><author><name sortKey="Hani, Khaled" sort="Hani, Khaled" uniqKey="Hani K" first="Khaled" last="Hani">Khaled Hani</name></author><author><name sortKey="Frere, Jacques" sort="Frere, Jacques" uniqKey="Frere J" first="Jacques" last="Frere">Jacques Frere</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23385143</idno><idno type="pmid">23385143</idno><idno type="doi">10.1016/j.resmic.2013.01.002</idno><idno type="wicri:Area/PubMed/Corpus">000632</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000632</idno><idno type="wicri:Area/PubMed/Curation">000628</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000628</idno><idno type="wicri:Area/PubMed/Checkpoint">000625</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000625</idno><idno type="wicri:Area/Main/Merge">000188</idno><idno type="wicri:Area/Main/Curation">000188</idno><idno type="wicri:Area/Main/Exploration">000188</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Diversity of legionellae strains from Tunisian hot spring water.</title><author><name sortKey="Ghrairi, Taoufik" sort="Ghrairi, Taoufik" uniqKey="Ghrairi T" first="Taoufik" last="Ghrairi">Taoufik Ghrairi</name><affiliation wicri:level="1"><nlm:affiliation>UR08-45, Department of Biochemistry, Faculty of Medicine of Sousse, 4002 Sousse, Tunisia. taoufik_ghrairi@yahoo.fr</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>UR08-45, Department of Biochemistry, Faculty of Medicine of Sousse, 4002 Sousse</wicri:regionArea><wicri:noRegion>4002 Sousse</wicri:noRegion></affiliation></author><author><name sortKey="Chaftar, Nawel" sort="Chaftar, Nawel" uniqKey="Chaftar N" first="Nawel" last="Chaftar">Nawel Chaftar</name></author><author><name sortKey="Jarraud, Sophie" sort="Jarraud, Sophie" uniqKey="Jarraud S" first="Sophie" last="Jarraud">Sophie Jarraud</name></author><author><name sortKey="Berjeaud, Jean Marc" sort="Berjeaud, Jean Marc" uniqKey="Berjeaud J" first="Jean Marc" last="Berjeaud">Jean Marc Berjeaud</name></author><author><name sortKey="Hani, Khaled" sort="Hani, Khaled" uniqKey="Hani K" first="Khaled" last="Hani">Khaled Hani</name></author><author><name sortKey="Frere, Jacques" sort="Frere, Jacques" uniqKey="Frere J" first="Jacques" last="Frere">Jacques Frere</name></author></analytic><series><title level="j">Research in microbiology</title><idno type="eISSN">1769-7123</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Typing Techniques (MeSH)</term><term>Biodiversity (MeSH)</term><term>Cluster Analysis (MeSH)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (genetics)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Electrophoresis, Gel, Pulsed-Field (MeSH)</term><term>Hot Springs (microbiology)</term><term>Hot Temperature (MeSH)</term><term>Legionella (classification)</term><term>Legionella (genetics)</term><term>Legionella (growth & development)</term><term>Legionella (isolation & purification)</term><term>Molecular Sequence Data (MeSH)</term><term>Molecular Typing (MeSH)</term><term>Phylogeny (MeSH)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Serotyping (MeSH)</term><term>Tunisia (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (composition chimique)</term><term>ADN bactérien (génétique)</term><term>ADN ribosomique (composition chimique)</term><term>ADN ribosomique (génétique)</term><term>ARN ribosomique 16S (génétique)</term><term>Analyse de regroupements (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Biodiversité (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Legionella (classification)</term><term>Legionella (croissance et développement)</term><term>Legionella (génétique)</term><term>Legionella (isolement et purification)</term><term>Phylogenèse (MeSH)</term><term>Réaction de polymérisation en chaine en temps réel (MeSH)</term><term>Sources thermales (microbiologie)</term><term>Sérotypie (MeSH)</term><term>Techniques de typage bactérien (MeSH)</term><term>Température élevée (MeSH)</term><term>Tunisie (MeSH)</term><term>Typage moléculaire (MeSH)</term><term>Électrophorèse en champ pulsé (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN bactérien</term><term>ADN ribosomique</term><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>ADN ribosomique</term><term>ARN ribosomique 16S</term><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Legionella</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Sources thermales</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hot Springs</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Biodiversity</term><term>Cluster Analysis</term><term>Electrophoresis, Gel, Pulsed-Field</term><term>Hot Temperature</term><term>Molecular Sequence Data</term><term>Molecular Typing</term><term>Phylogeny</term><term>Real-Time Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term><term>Serotyping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term><term>Analyse de séquence d'ADN</term><term>Biodiversité</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sérotypie</term><term>Techniques de typage bactérien</term><term>Température élevée</term><term>Tunisie</term><term>Typage moléculaire</term><term>Électrophorèse en champ pulsé</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to evaluate the frequency of Legionella pneumophila in three hot spring water recreation areas by conventional culture on GVPC and by Real Time-PCR. No legionellae were isolated from the spring water upstream the distribution system suggesting strongly that the thermal area was the probable source of these pathogens. L. pneumophila was present at high level in some samples, reaching values of 8.2 × 10(3) CFU/l at the site C. We observed a low diversity among the 18 isolated Legionella strains according to 16S DNA analysis, since only L. pneumophila and Legionella londiniensis were identified. All of the L. pneumophila strains belong to five serogroups (1, 4, 5, 6 and 8). Pulsed-field gel electrophoresis analysis identified various genotypes among these serogroups and showed that these strains had pulsotypes distinct from L. pneumophila present in the CNRL Legionella-database. These environmental strains showed higher tolerance to heat shock stress at 48 °C than L. pneumophila clinical strain, which may explain their presence in warm waters. Our data suggested that potential pathogen legionellae are present in Tunisian medicalized thermal spas and emphasized the importance of adopting control measures to prevent infection.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Berjeaud, Jean Marc" sort="Berjeaud, Jean Marc" uniqKey="Berjeaud J" first="Jean Marc" last="Berjeaud">Jean Marc Berjeaud</name><name sortKey="Chaftar, Nawel" sort="Chaftar, Nawel" uniqKey="Chaftar N" first="Nawel" last="Chaftar">Nawel Chaftar</name><name sortKey="Frere, Jacques" sort="Frere, Jacques" uniqKey="Frere J" first="Jacques" last="Frere">Jacques Frere</name><name sortKey="Hani, Khaled" sort="Hani, Khaled" uniqKey="Hani K" first="Khaled" last="Hani">Khaled Hani</name><name sortKey="Jarraud, Sophie" sort="Jarraud, Sophie" uniqKey="Jarraud S" first="Sophie" last="Jarraud">Sophie Jarraud</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Ghrairi, Taoufik" sort="Ghrairi, Taoufik" uniqKey="Ghrairi T" first="Taoufik" last="Ghrairi">Taoufik Ghrairi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000188 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000188 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23385143
|texte= Diversity of legionellae strains from Tunisian hot spring water.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23385143" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |